The Effect of Tuna Fish Oil (Thunnus albacares) on the Total Cholesterol, LDL Cholesterol, HDL Cholesterol and the Triacylglycerol Level on Hypercholesterolemia Rats (Rattus norvegicus)

Tangka, Juliet and Banne, Yos and Dumanauw, Jovie M. and Rumagit, Benedicta I. (2016) The Effect of Tuna Fish Oil (Thunnus albacares) on the Total Cholesterol, LDL Cholesterol, HDL Cholesterol and the Triacylglycerol Level on Hypercholesterolemia Rats (Rattus norvegicus). International Journal of Pharmaceutical and Clinical Research, 8 (5). ISSN ISSN- 0975 1556

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Abstract

Setting a daily diet is the first step in tackling the risk of CHD, which is due to the increasing levels of cholesterol and fat. This can be done by consuming fish that is a natural source of ω-3 fatty acids, EPA and DHA. One of the products processed from the extraction of fish, especially fatty fish is tuna fish oil that almost 40% of the total fat content is ω-3 PUFAs are EPA and DHA. This study aimed to determine the effect of Tuna fish oil (Thunnus albacares) against the total cholesterol, LDL cholesterol, HDL cholesterol and triacylglycerol levels in the blood of hypercholesterolemia rats (Rattus norvegicus). The fish oil was extracted from fish waste Tuna with a reflux method using chloroform, and the levels of EPA and DHA were determined using a gas liquid chromatography. The samples were 40 male rats. Five rats were used as a normal control group, and 35 rats were subsequently given a high-cholesterol diet for 6 weeks to achieve a state of hypercholesterolemia. Five rats from the experiment group were taken as data for hypercholesterolemia. Of 35 rats, 30 were divided into 3 groups of 10 rats: 1) a control posttest group (P0), receiving the treatment of a high-cholesterol diet, 2) the first treatment group (P1), which was given a high-cholesterol diet + 1 dose (0.5 ml) of Tuna fish oil and 3) the second treatment group (P2), which was given a high-cholesterol diet + 2 doses (1 ml) of Tuna fish oil. The treatment was given for four weeks and the data were then analyzed using descriptive statistics and ANOVA test, followed by LSD test. The results showed that the Tuna fish oil supplementation can reduce the total cholesterol, the LDL cholesterol and the triacylglycerol levels significantly with p <0.05. It can also increase the HDL cholesterol levels significantly with p <0.05.

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