Comparisson Of The Examination Of Mycobacterium Tuberculosis (M.Tb) Using Polymerase Chain Reaction (PCR) And ZiehlNeelsen (ZN) On Tuberculosis (Tb) Patients Suspect In Public Health Facilities Of Manado

Rambi, Elne Vieke and konoralma, Ketrina and Sumampouw, Jonas E. and Tumbol, Michael Vallery Loueis (2022) Comparisson Of The Examination Of Mycobacterium Tuberculosis (M.Tb) Using Polymerase Chain Reaction (PCR) And ZiehlNeelsen (ZN) On Tuberculosis (Tb) Patients Suspect In Public Health Facilities Of Manado. International Conference on Medical Laboratory Technology, 2 (1). pp. 69-74.

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Abstract

(TB) is an infectious disease caused by Mycobacterium tuberculosis. TB infections occur through the air, namely the inhalation of droplets containing germs from an infected person. Four People exposed with Mycobacterium tuberculosis have a 10% risk of developing pulmonary TB. The aim of this research was to compare of the examination of M.Tb using polymerase chain reaction and ziehlneelsen on TB Patients suspect in Public Health Center of Manado. The research method used was analytic laboratory examination. As the result, TB examination using ZiehlNeelsen method found 13 samples with positive TB, while using the PCR examination method 93 samples (93,6%) showed positive. There was significantly differences between PCR and ZiehlNeelsen examination method. TB examination using the PCR method had high sensitivity and specificity to detect Mycobacterium tuberculosis, so that more MTB bacteria can be detected than using the ZN test. It is recommended that health workers at the Public Health center to diagnose TB should use an examination that has high sensitivity and specificity so that the results obtained are more accurate.

Item Type: Article
Subjects: Penelitian
Depositing User: Admin Poltekkes
Date Deposited: 27 Apr 2023 14:41
Last Modified: 27 Apr 2023 14:41
URI: http://repository.poltekkes-manado.ac.id/id/eprint/1073

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